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Betacellulin Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is P35070.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 μg | $113 | 7-10 days | |
50 μg | $339 | 7-10 days |
Biological Activity | ED 50 < 4.0 pg/ml, measured in a cell proliferation assay using 3T3 cells. |
Description | Betacellulin Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is P35070. |
Species | Human |
Expression System | HEK293 Cells |
Tag | Tag Free |
Accession Number | P35070 |
Synonyms | BTC |
Construction | Asp32-Tyr111 |
Protein Purity | > 95% as determined by SDS-PAGE |
Molecular Weight | 15~18 kDa (Reducing conditions) |
Endotoxin | < 0.2 EU/μg of protein as determined by the LAL method. |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Betacellulin (BTC) is a member of the EGF family of growth factors that also includes EGF, TGF-α, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin, Heregulin and Neuregulins. Mature human BTC protein exhibits 80% amino acidsimilarity with mouse BTC protein. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. It is synthesized primarily as a transmembrane precursor, which is then processed to a mature molecule by proteolytic events. BTC signals through the EGF receptor. |
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