ATP5F1B Protein, Human, Recombinant (His & Myc & SUMO) is expressed in E. coli.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
20 μg | 20 days | $ 198.00 | |
100 μg | 20 days | $ 389.00 | |
1 mg | 20 days | $ 1,680.00 |
Description | ATP5F1B Protein, Human, Recombinant (His & Myc & SUMO) is expressed in E. coli. |
Species | Human |
Expression System | E. coli |
Tag | N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged |
Accession Number | P06576 |
Synonyms | ATP5B, ATP synthase F1 subunit beta, ATP5F1B, ATPSB, ATP synthase subunit beta, mitochondrial, ATPMB |
Amino Acid | YSVFAGVGERTREGNDLYHEMIESGVINL kDaTSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
Construction | 230-529 aa |
Protein Purity | > 85% as determined by SDS-PAGE. |
Molecular Weight | 52.8 kDa (predicted) |
Formulation | Tris-based buffer,50% glycerol |
Reconstitution | A hardcopy of COA with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
ATP5F1B Protein, Human, Recombinant (His & Myc & SUMO) ATP5B ATP synthase F1 subunit beta ATP5F1B ATPSB ATP synthase subunit beta, mitochondrial ATPMB recombinant recombinant-proteins proteins protein