ARMET/MANF Protein, Human, Recombinant (His) V2

Name: ARMET/MANF Protein, Human, Recombinant (His) V2
Brand: TargetMol
SKU: TMPY-01678U
Rating: 4.5 (1 reviews)

(Synonyms: mesencephalic astrocyte-derived neurotrophic factor, ARP, ARMET) Copy Product Info

Synonyms: mesencephalic astrocyte-derived neurotrophic factor, ARP, ARMET

Catalog No. TMPY-01678U Copy Product Info

ARMET/MANF Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P55145.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$56	7-10 days	7-10 days
10 µg	$88	7-10 days	7-10 days
20 µg	$142	7-10 days	7-10 days
50 µg	$276	7-10 days	7-10 days
100 µg	$462	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity has not been tested. It is theoretically active, but we cannot guarantee it.
Description	ARMET/MANF Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P55145.
Species	Human
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	P55145
Construction	Leu25-Leu182
Protein Purity	> 95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	mesencephalic astrocyte-derived neurotrophic factor, ARP, ARMET
Research Background	Mesencephalic astrocyte-derived neurotrophic factor(MANF) is a secreted protein which belongs to the ARMET family. MANF selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. MANF enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. MANF inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF and a homologous protein, the conserved dopamine neurotrophic factor (CDNF), form a novel evolutionary conserved family of neurotrophic factors. MANF expression was widespread in the nervous system and non-neuronal tissues.

Chemical Properties

Molecular Weight

18.99 kDa (Predicted); 17 kDa (Reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.