AMCase/CHIA Protein, Mouse, Recombinant (His)

Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 371.00
500 μg	5 days	$ 1,490.00
1 mg	5 days	$ 2,480.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 371.00

500 μg

5 days

$ 1,490.00

1 mg

5 days

$ 2,480.00

Description

Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q91XA9
Synonyms	CHIT2, Chia1, AMCase, YNL, Acidic mammalian chitinase, CHIA, EC 3.2.1.14
Construction	Tyr22-Pro473
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	51 kDa (predicted). Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.

Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.