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Synonyms: PLAP-1, PLAP, ALPP, Alkaline phosphatase


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $108 | 7-10 days | 7-10 days | |
| 10 µg | $169 | 7-10 days | 7-10 days | |
| 20 µg | $267 | 7-10 days | 7-10 days | |
| 50 µg | $488 | 7-10 days | 7-10 days | |
| 100 µg | $776 | 7-10 days | 7-10 days | |
| 200 µg | $1,230 | 7-10 days | 7-10 days | |
| 500 µg | $2,290 | 7-10 days | 7-10 days |
| Bioactivity | Loaded Anti-Alkaline Phosphatase Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus ALPP, His Tag with an affinity constant of 0.13 µM as determined in BLI assay . |
| Description | Alkaline Phosphatase/ALPP Protein, Cynomolgus, Recombinant (His), Biotinylated is expressed in HEK293 Cells. The accession number is XP_045223825.1. |
| Species | Cynomolgus |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | XP_045223825.1 |
| Construction | Ile21-Thr503 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in 25 mM Tris, 100 mM NaCl, 1 mM MgCl2(pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | PLAP-1, PLAP, ALPP, Alkaline phosphatase |
| Research Background | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
| Molecular Weight | 53.69 kDa (Predicted); 57-70 kDa (Reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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