15 Lipoxygenase 2 Protein, Human, Recombinant

Name: 15 Lipoxygenase 2 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-03553
Rating: 4.5 (1 reviews)

(Synonyms: arachidonate 15-lipoxygenase, type B, ALOX15B, 15-LOX-2) Copy Product Info

Synonyms: arachidonate 15-lipoxygenase, type B, ALOX15B, 15-LOX-2

Catalog No. TMPY-03553 Copy Product Info

15 Lipoxygenase 2 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 76 kDa and the accession number is AAH35217.1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$85	7-10 days	7-10 days
10 µg	$146	7-10 days	7-10 days
20 µg	$242	7-10 days	7-10 days
50 µg	$477	7-10 days	7-10 days
100 µg	$929	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	15 Lipoxygenase 2 Protein, Human, Recombinant is expressed in Baculovirus insect cells. The predicted molecular weight is 76 kDa and the accession number is AAH35217.1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	Tag Free
Accession Number	AAH35217.1
Construction	A DNA sequence encoding the human ALOX15B (AAH35217.1)( Met1-Ile676) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. Predicted N terminal: Gly
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	arachidonate 15-lipoxygenase, type B, ALOX15B, 15-LOX-2
Research Background	ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. ALOX15B converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. ALOX15B gene is located in a cluster of related genes and a pseudogene that spans approximately 1 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.

Chemical Properties

Molecular Weight

76 kDa (predicted); 62-66 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.