SARS-CoV-2 Nucleocapsid Protein (S194L, His)

Name: SARS-CoV-2 Nucleocapsid Protein (S194L, His)
Brand: TargetMol
SKU: TMPY-06097
Rating: 4.5 (1 reviews)

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Catalog No. TMPY-06097 Copy Product Info

SARS-CoV-2 Nucleocapsid Protein (S194L, His) is expressed in E. coli expression system. The predicted molecular weight is 46.6 kDa and the accession number is P0DTC9.

Pack Size	Price	USA Stock	Global Stock
5 µg	$77	7-10 days	7-10 days
10 µg	$123	7-10 days	7-10 days
20 µg	$198	7-10 days	7-10 days
50 µg	$397	7-10 days	7-10 days
100 µg	$793	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	SARS-CoV-2 Nucleocapsid Protein (S194L, His) is expressed in E. coli expression system. The predicted molecular weight is 46.6 kDa and the accession number is P0DTC9.
Species	SARS-CoV-2
Expression System	E. coli
Tag	N-His
Accession Number	P0DTC9
Construction	A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (YP_009724397.2) (Met1-Ala419(S194L)) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: Met
Protein Purity	> 85 % as determined by SDS-PAGE.
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM PB, 500 mM NaCl, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Research Background	Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Chemical Properties

Molecular Weight

46.6 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.