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PCNA Nuclear Loading Control Polyclonal Antibody
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Catalog No. TMAB-01343
PCNA Nuclear Loading Control Polyclonal Antibody is a Rabbit antibody targeting PCNA Nuclear Loading Control. PCNA Nuclear Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
PCNA Nuclear Loading Control Polyclonal Antibody
PCNA Nuclear Loading Control Polyclonal Antibody
😃Good
Catalog No. TMAB-01343
PCNA Nuclear Loading Control Polyclonal Antibody is a Rabbit antibody targeting PCNA Nuclear Loading Control. PCNA Nuclear Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $127 | 7-10 days | |
| 100 μL | $183 | 7-10 days | |
| 500 μL | $754 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | PCNA Nuclear Loading Control Polyclonal Antibody is a Rabbit antibody targeting PCNA Nuclear Loading Control. PCNA Nuclear Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 μg total protein per Lane of various lysates probed with PCNA polyclonal antibody, unconjugated (TMAB-01343) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 15. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 16. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 17. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 18. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 19. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 20. Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA Polyclonal Antibody, Unconjugated (TMAB-01343) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
| Recommended Dose | WB: 1:1000-10000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; ICC/IF: 1:100-500; IF: 1:200-1000; FCM: 1ug/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Note=Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure. |
| Immunogen | KLH conjugated synthetic peptide: mouse PCNA |
| Antigen Species | Mouse |
| Gene ID | |
| Biology Area | Cell division,Tumor antigens,Markers,DNA / RNA binding,DNA Synthesis,Methylated DNA Binding,PCNA,Replication |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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