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GAPDH Loading Control Antibody (8S504)
😃Good
Catalog No. TMAY-00017
Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
GAPDH Loading Control Antibody (8S504) is a Mouse antibody targeting GAPDH. GAPDH Loading Control Antibody (8S504) can be used in WB,IHC-P.
GAPDH Loading Control Antibody (8S504)
GAPDH Loading Control Antibody (8S504)
😃Good
Catalog No. TMAY-00017Alias HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD
GAPDH Loading Control Antibody (8S504) is a Mouse antibody targeting GAPDH. GAPDH Loading Control Antibody (8S504) can be used in WB,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $186 | 7-10 days |
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Product Introduction
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Chemical Properties
| Description | GAPDH Loading Control Antibody (8S504) is a Mouse antibody targeting GAPDH. GAPDH Loading Control Antibody (8S504) can be used in WB,IHC-P. |
| Alias | HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, GAPD, G3PD |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 8S504 |
| Reactivity | Human |
| Verified Activity | 1. Anti-GAPDH mouse monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: Hela Whole Cell Lysate. -Lane C: MCF7 Whole Cell Lysate. -Lane D: MCF7 whole cell Lysate. -Lane E: PC-3 whole cell Lysate. -Lane F: Daudi whole cell Lysate. -Lane G: A549 whole cell Lysate. -Lane H: Rat heart tissue Lysate. -Lane I: Rat liver tissue Lysate. -Lane J: Rat spleen tissue Lysate. -Lane K: Rat lung tissue Lysate. -Lane L: Rat kidney tissue Lysate. -Lane M: Rat brain tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size: kDa. -Observed band size:38 kDa. 2. Immunochemical staining of human GAPDH in human renal carcinoma with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human GAPDH in human cirrhosis with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. |
| Application | WB,IHC-P |
| Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human GAPDH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide corresponding to the center region of the human GAPDH |
| Antigen Species | Human |
| Biology Area | Adaptor Proteins |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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