Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.

Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated is expressed in CHO Stable Cells. The accession number is P22748-1.

Human

CHO Cells

RP17,Car4,CAIV

A DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.

≥ 90% as determined by SDS-PAGE.

Please refer to the lot-specific COA.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3-dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.