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Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated

(Synonyms: RP17, Car4, CAIV) Copy Product Info

Synonyms: RP17, Car4, CAIV

Catalog No. TMPY-07021 Copy Product Info
Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated is expressed in CHO Stable Cells. The accession number is P22748-1.
Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$2087-10 days7-10 days
10 µg$3437-10 days7-10 days
20 µg$5887-10 days7-10 days
50 µg$9397-10 days7-10 days
100 µg$1,6007-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated is expressed in CHO Stable Cells. The accession number is P22748-1.
Species
Human
Expression System
CHO Cells
TagC-His
Accession NumberP22748-1
ConstructionA DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Protein Purity
≥ 90% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
ReconstitutionPlease refer to the lot-specific COA.
SynonymsRP17, Car4, CAIV
Research Background
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3-dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.
Chemical Properties
Molecular Weight31.69 kDa (predicted); 33 kDa (reducing contition)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated chemical structure | Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated molecular weight