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Beta-Tubulin Loading Control Polyclonal Antibody is a Rabbit antibody targeting Beta-Tubulin Loading Control. Beta-Tubulin Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $126 | 7-10 days | |
100 μL | $182 | 7-10 days | |
500 μL | $756 | 7-10 days |
Description | Beta-Tubulin Loading Control Polyclonal Antibody is a Rabbit antibody targeting Beta-Tubulin Loading Control. Beta-Tubulin Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Alias | Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (TMAB-00236) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Sample: Lane 1,3,5: Mouse Cerebellum tissue lysates Lane 2,4,6: Rat Cerebellum tissue lysates Primary: Anti-Beta tubulin (TMAB-00236) at 1/10000~1/50000 dilution Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 48 kDa 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:10000-100000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasmic, cytoskeleton. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] |
Immunogen | KLH conjugated synthetic peptide: human tubulin Beta |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Microtubules,Tubulin |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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