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Beta-Tubulin Loading Control Polyclonal Antibody

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Catalog No. TMAB-00236
Alias Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB

Beta-Tubulin Loading Control Polyclonal Antibody is a Rabbit antibody targeting Beta-Tubulin Loading Control. Beta-Tubulin Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Beta-Tubulin Loading Control Polyclonal Antibody

Beta-Tubulin Loading Control Polyclonal Antibody

😃Good
Catalog No. TMAB-00236Alias Tubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB
Beta-Tubulin Loading Control Polyclonal Antibody is a Rabbit antibody targeting Beta-Tubulin Loading Control. Beta-Tubulin Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$1267-10 days
100 μL$1827-10 days
500 μL$7567-10 days
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Product Introduction

Bioactivity
Description
Beta-Tubulin Loading Control Polyclonal Antibody is a Rabbit antibody targeting Beta-Tubulin Loading Control. Beta-Tubulin Loading Control Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasTubulin beta-5 chain, Tubulin beta chain, TUBB5, TUBB
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (TMAB-00236)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Sample:
Lane 1,3,5: Mouse Cerebellum tissue lysates
Lane 2,4,6: Rat Cerebellum tissue lysates
Primary: Anti-Beta tubulin (TMAB-00236) at 1/10000~1/50000 dilution
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 48 kDa
3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beta tubulin Polyclonal Antibody, Unconjugated (TMAB-00236) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:10000-100000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic, cytoskeleton.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human tubulin Beta
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Microtubules,Tubulin
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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