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Beta-Tubulin Loading Control Antibody (8X221)

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Catalog No. TMAY-00014

Beta-Tubulin Loading Control Antibody (8X221) is a Mouse antibody targeting Beta-Tubulin. Beta-Tubulin Loading Control Antibody (8X221) can be used in WB,IHC-P,ICC/IF,IP.

Beta-Tubulin Loading Control Antibody (8X221)

Beta-Tubulin Loading Control Antibody (8X221)

😃Good
Catalog No. TMAY-00014
Beta-Tubulin Loading Control Antibody (8X221) is a Mouse antibody targeting Beta-Tubulin. Beta-Tubulin Loading Control Antibody (8X221) can be used in WB,IHC-P,ICC/IF,IP.
Pack SizePriceAvailabilityQuantity
20 μL$69 7-10 days
100 μL$139 7-10 days
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Instruction Manual of Detection Antibody
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Mouse Monoclonal

Application: WB, IHC-P, ICC/IF, IP

Reactivity: Human
Ig Type
Mouse IgG2b
Clone
8X221
Reactivity
Human
Verified Activity
1. Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
2. Immunochemical staining of human Tubulin in human malignant melanoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
3. Immunochemical staining of human Tubulin in human prostate with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).
4. Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.
5. Tubulin was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg HepG2 Whole Cell Lysate.
-4 µL anti-Tubulin mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
-Primary antibody:
-Anti-Tubulin mouse monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size: 55 kDa.
-Observed band size:55 kDa.
6. Anti-Beta-Tubulin mouse monoclonal antibody at 1:10000, 1:20000, 1:50000, 1:100000 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: Hela Whole Cell Lysate.
-Lane C: Raw246.7 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:50 kDa.
-Observed band size:54 kDa.
7. Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution.
-Lane A: Rabbit heart tissue Lysate.
-Lane B: Rabbit liver tissue Lysate.
-Lane C: Rabbit spleen tissue Lysate.
-Lane D: Rabbit lung tissue Lysate.
-Lane E: Rabbit kidney tissue Lysate.
-Lane F: Rabbit brain tissue Lysate.
-Lane G: Rabbit muscle tissue Lysate.
-Lane H: Rabbit stomach tissue Lysate.
-Lane I: Rabbit small intestine tissue lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:50 kDa.
-Observed band size:54 kDa.
8. Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution.
-Lane A: Rat heart tissue Lysate.
-Lane B: Rat liver tissue Lysate.
-Lane C: Rat spleen tissue Lysate.
-Lane D: Rat lung tissue Lysate.
-Lane E: Rat kidney tissue Lysate.
-Lane F: Rat muscle tissue Lysate.
-Lane G: Rat stomach tissue Lysate.
-Lane H: Rat small intestine tissue lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:50 kDa.
-Observed band size:54 kDa.
9. Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution.
-Lane A: Mouse heart tissue Lysate.
-Lane B: Mouse liver tissue Lysate.
-Lane C: Mouse spleen tissue Lysate.
-Lane D: Mouse lung tissue Lysate.
-Lane E: Mouse kidney tissue Lysate.
-Lane F: Mouse brain tissue Lysate.
-Lane G: Mouse muscle tissue Lysate.
-Lane H: Mouse stomach tissue Lysate.
-Lane I: Mouse pancreas tissue lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:50 kDa.
-Observed band size:54 kDa.
10. Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution.
-Lane A: Jurkat Whole Cell Lysate.
-Lane B: 293T Whole Cell Lysate.
-Lane C: K562 Whole Cell Lysate.
-Lane D: Hela Whole Cell Lysate.
-Lane E: RAW264.7 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:50 kDa.
-Observed band size:54 kDa.
11. Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: Daudi Whole Cell Lysate.
-Lane C: MOLT-4 Whole Cell Lysate.
-Lane D: A549 Whole Cell Lysate.
-Lane E: 293T Whole Cell Lysate.
-Lane F: HelaS3 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:50 kDa.
-Observed band size:54 kDa
Application
WB,IHC-P,ICC/IF,IP
Recommended Dose
WB: 1:1000-1:100000; IHC-P: 1/2000-1/20000; ICC-IF: 1:50-1:200; IP: 5-10 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis product is a recombinant monoclonal antibody expressed from HEK293 cells.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundBeta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: N-terminus of the human beta-tubulin
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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