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Beta-Actin Loading Control Antibody (9Q394)
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Catalog No. TMAY-00015
Beta-Actin Loading Control Antibody (9Q394) is a Rabbit antibody targeting Beta-Actin. Beta-Actin Loading Control Antibody (9Q394) can be used in WB,IP.
Beta-Actin Loading Control Antibody (9Q394)
Beta-Actin Loading Control Antibody (9Q394)
😃Good
Catalog No. TMAY-00015
Beta-Actin Loading Control Antibody (9Q394) is a Rabbit antibody targeting Beta-Actin. Beta-Actin Loading Control Antibody (9Q394) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $46 | 7-10 days | |
| 100 μL | $140 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Ig Type | Rabbit IgG |
| Clone | 9Q394 |
| Reactivity | Human |
| Verified Activity | 1. Anti-beta-Actin rabbit monoclonal antibody at 1:20000 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: HeLa Whole Cell Lysate. -Lane C: HepG2 Whole Cell Lysate. -Lane D: A549 Whole Cell Lysate. -Lane E: Mouse brain tissue Lysate. -Lane F: Mouse skeletal muscle tissue Lysate. -Lane G: Mouse kidney tissue Lysate. -Lane H: Rat brain tissue Lysate. -Lane I: Rat lung tissue Lysate. -Lane J: Rat kidney tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:42 kDa. -Observed band size:42 kDa. 2. Actin was immunoprecipitated using: -Lane A:0.5 mg HeLa Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate. -Lane C:0.5 mg MCF7 Whole Cell Lysate. -Lane D:0.5 mg A431 Whole Cell Lysate. -4 µL anti-Actin rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-Actin rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 43 kDa. -Observed band size:44 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:10000-1:100000; IP: 1-5 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | It is a chimeric monoclonal antibody combining the constant domains of the rabbit IgG molecule with mouse variable regions. The variable region was obtained from a mouse immunized with A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB. The antibody was produced using recombinant antibody technology. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: N-terminus of the Human beta Actin / ACTB |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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