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Anti-ZNRF3 Polyclonal Antibody
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Catalog No. TMAB-14450
Anti-ZNRF3 Polyclonal Antibody is a Rabbit antibody targeting ZNRF3. Anti-ZNRF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ZNRF3 Polyclonal Antibody
Anti-ZNRF3 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-14450
Anti-ZNRF3 Polyclonal Antibody is a Rabbit antibody targeting ZNRF3. Anti-ZNRF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-ZNRF3 Polyclonal Antibody is a Rabbit antibody targeting ZNRF3. Anti-ZNRF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZNRF3) Polyclonal Antibody, Unconjugated (TMAB-14450) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Human HepG2 cell Lysates Lane 2: Human A549 cell Lysates Lane 3: Human U-2 OS cell Lysates Primary: Anti-ZNRF3 (TMAB-14450) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 95 kDa Observed band size: 120 kDa 3. 25 μg total protein per lane of various lysates (see on figure) probed with ZNRF3 polyclonal antibody, unconjugated (TMAB-14450) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). ZNRF3 (Zinc/RING finger protein 3), also known as RNF203 (RING finger protein 203), is a 936 amino acid single pass transmembrane protein that contains one RING-type zinc finger. Related zinc/RING finger proteins, such as ZNRF1 and ZNRF2, are E3 ubiquitin-protein ligases that are thought to be involved in the establishment and maintenance of neuronal transmission and plasticity, therefore it is likely that ZNRF3 may function in a similar manner. |
| Immunogen | KLH conjugated synthetic peptide: human ZNRF3 |
| Antigen Species | Human |
| Gene Name | ZNRF3 |
| Gene ID | |
| Protein Name | E3 ubiquitin-protein ligase ZNRF3 |
| Uniprot ID | |
| Function | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. |
| Molecular Weight | Theoretical: 95 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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