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Anti-ZNF307/ZKSCAN3 Polyclonal Antibody is a Rabbit antibody targeting ZNF307/ZKSCAN3. Anti-ZNF307/ZKSCAN3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-ZNF307/ZKSCAN3 Polyclonal Antibody is a Rabbit antibody targeting ZNF307/ZKSCAN3. Anti-ZNF307/ZKSCAN3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
Alias | ZSCAN35, ZSCAN13, ZNF309, ZKSCAN4, ZKSCAN3, ZKSC4, ZKSC3, Zinc finger with KRAB and SCAN domains 4, zinc finger with KRAB and SCAN domains 4, Zinc finger with KRAB and SCAN domains 3, Zinc finger protein zfp47, Zinc finger protein with KRAB and SCAN domains 3, Zinc finger protein 47 homolog, Zinc finger protein 309, Zinc finger protein 307, Zinc finger protein 306, isoform CRA_a, Zinc finger protein 306, Zinc finger and SCAN domain-containing protein 13, Zfp-47, Zfp47, Zfp307, ZFP306, Zf47, Skz1, SCAN-KRAB-zinc finger protein, p373c6.1(novel C2H2 type zinc finger protein), p373c6.1, P373c6.1, P1P373C6, mszf35, FLJ32136, dJ874C20.1 |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit) |
Verified Activity | 1. Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated (TMAB-01996) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: mouse fetal liver; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated (TMAB-01996) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated (TMAB-01996) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ZNF307 Polyclonal Antibody, Unconjugated (TMAB-01996) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Sample: K562 (Human) Cell Lysate at 40 μg Primary: Anti-ZNF307/ZKSCAN3 (TMAB-01996) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa 6. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ZNF307/ZKSCAN3) polyclonal Antibody, Unconjugated (TMAB-01996) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 7. Blank control (black line): SH-SY5Y. Primary Antibody (green line): Rabbit Anti-ZNF307/ZKSCAN3 antibody (TMAB-01996) Dilution: 2 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 2ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide: human ZNF307 |
Antigen Species | Human |
Gene ID | |
Uniprot ID |
Molecular Weight | Theoretical: 62 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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