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Anti-ZHX2 Polyclonal Antibody
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Catalog No. TMAB-14315
Anti-ZHX2 Polyclonal Antibody is a Rabbit antibody targeting ZHX2. Anti-ZHX2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Anti-ZHX2 Polyclonal Antibody
Anti-ZHX2 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-14315
Anti-ZHX2 Polyclonal Antibody is a Rabbit antibody targeting ZHX2. Anti-ZHX2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-ZHX2 Polyclonal Antibody is a Rabbit antibody targeting ZHX2. Anti-ZHX2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat thyroid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZHX2) Polyclonal Antibody, Unconjugated (TMAB-14315) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZHX2) Polyclonal Antibody, Unconjugated (TMAB-14315) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ZHX2) Polyclonal Antibody, Unconjugated (TMAB-14315) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ZHX2) polyclonal Antibody, Unconjugated (TMAB-14315) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Ubiquitously expressed. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq, Jul 2008].[FUNCTION] Acts as a transcriptional repressor.[SUBUNIT] Forms homodimers. Also forms heterodimers with ZHX1 and ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts with NFYA.[SUBCELLULAR LOCATION] Nucleus. [TISSUE SPECIFICITY] Ubiquitously expressed. [SIMILARITY] Belongs to the ZHX family. |
| Immunogen | KLH conjugated synthetic peptide: human ZHX2 |
| Antigen Species | Human |
| Gene Name | ZHX2 |
| Gene ID | |
| Protein Name | Zinc fingers and homeoboxes protein 2 |
| Uniprot ID | |
| Function | Acts as a transcriptional repressor. |
| Molecular Weight | Theoretical: 92 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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