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Anti-ZHX2 Polyclonal Antibody

Catalog No. TMAB-14315 Copy Product Info
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Anti-ZHX2 Polyclonal Antibody is a Rabbit antibody targeting ZHX2. Anti-ZHX2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

Anti-ZHX2 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-14315

Anti-ZHX2 Polyclonal Antibody is a Rabbit antibody targeting ZHX2. Anti-ZHX2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

Anti-ZHX2 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-ZHX2 Polyclonal Antibody is a Rabbit antibody targeting ZHX2. Anti-ZHX2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat thyroid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZHX2) Polyclonal Antibody, Unconjugated (TMAB-14315) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZHX2) Polyclonal Antibody, Unconjugated (TMAB-14315) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ZHX2) Polyclonal Antibody, Unconjugated (TMAB-14315) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ZHX2) polyclonal Antibody, Unconjugated (TMAB-14315) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityUbiquitously expressed.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq, Jul 2008].[FUNCTION] Acts as a transcriptional repressor.[SUBUNIT] Forms homodimers. Also forms heterodimers with ZHX1 and ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts with NFYA.[SUBCELLULAR LOCATION] Nucleus. [TISSUE SPECIFICITY] Ubiquitously expressed. [SIMILARITY] Belongs to the ZHX family.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ZHX2
Antigen Species
Human
Gene Name
ZHX2
Gene ID
Protein Name
Zinc fingers and homeoboxes protein 2
Uniprot ID
Function
Acts as a transcriptional repressor.
Chemical Properties
Molecular WeightTheoretical: 92 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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