Anti-ZFYVE26 Polyclonal Antibody is a Rabbit antibody targeting ZFYVE26. Anti-ZFYVE26 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZFYVE26) Polyclonal Antibody, Unconjugated (TMAB-14312) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-ZFYVE26 (TMAB-14312) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 285 kD
Observed band size: 285 kD
3. Sample:
Brain (Mouse) Lysate at 40 μg
U251 (Human) CellLysate at 30 μg
U87MG (Human) CellLysate at 30 μg
Primary: Anti-ZFYVE26 (TMAB-14312) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 285 kD
Observed band size: 285 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ZFYVE26

Human

ZFYVE26

Zinc finger FYVE domain-containing protein 26

Phosphatidylinositol 3-phosphate-binding protein required for the abcission step in cytokinesis: recruited to the midbody during cytokinesis and acts as a regulator of abcission. May also be required for efficient homologous recombination DNA double-strand break repair.