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Anti-ZFP30 Polyclonal Antibody 2

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Catalog No. TMAB-14294

Anti-ZFP30 Polyclonal Antibody 2 is a Rabbit antibody targeting ZFP30. Anti-ZFP30 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

Anti-ZFP30 Polyclonal Antibody 2

Anti-ZFP30 Polyclonal Antibody 2

Copy Product Info
😃Good
Catalog No. TMAB-14294
Anti-ZFP30 Polyclonal Antibody 2 is a Rabbit antibody targeting ZFP30. Anti-ZFP30 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-ZFP30 Polyclonal Antibody 2 is a Rabbit antibody targeting ZFP30. Anti-ZFP30 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZFP30) Polyclonal Antibody, Unconjugated (TMAB-14294) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: MDA-MB-231 (Human) Cell Lysate at 30 μg
Primary: Anti-ZFP30 (TMAB-14294) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
3. Sample:
293T (Human) Cell Lysate at 30 μg
U251 (Human) Cell Lysate at 30 μg
U87Mg (Human) Cell Lysate at 30 μg
MDA-MB-231 (Human) Cell Lysate at 30 μg
Primary: Anti-ZFP30 (TMAB-14294) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundZFP30 is a 519 amino acid protein that localizes to the nucleus and is composed of thirteen C2H2-type zinc fingers and one KRAB domain. ZFP30 is encoded by a gene located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG families, and Fc receptors (FcRs).
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ZFP30
Antigen Species
Human
Gene Name
Zfp30
Gene ID
Protein Name
Zinc finger protein 30 homolog
Uniprot ID
Function
May be involved in transcriptional regulation.
Chemical Properties
Molecular WeightTheoretical: 62 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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