Anti-ZEB1 Antibody (5M128) is a Mouse antibody targeting ZEB1. Anti-ZEB1 Antibody (5M128) can be used in WB,FCM.

IgG1, k

5M128

Human

1. Blank control: A549.
Primary Antibody (green line): Mouse Anti-ZEB1 antibody (TMAB-14284)
Dilution: 1:50;
Secondary Antibody: Goat anti-mouse IgG-PE
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Human A549 cell lysates
Primary: Anti-ZEB1 (TMAB-14284) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 100 kD

WB: 1:500-2000; FCM: 1:10-50

Monoclonal

KLH conjugated synthetic peptide: human ZEB1

Human

ZEB1

Zinc finger E-box-binding homeobox 1

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.