Anti-ZDHHC6 Polyclonal Antibody is a Rabbit antibody targeting ZDHHC6. Anti-ZDHHC6 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Pig,Horse)

1. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-ZDHHC6 (TMAB-01994) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
2. Sample:
Lane 1: Mouse Pancreas tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Mouse Raw264.7 cell lysates
Lane 5: Mouse NIH/3T3 cell lysates
Lane 6: Rat Kidney tissue lysates
Lane 7: Rat Cerebrum tissue lysates
Lane 8: Rat Testis tissue lysates
Lane 9: Human HL-60 cell lysates
Primary: Anti-ZDHHC6 (TMAB-01994) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ZDHHC6) Polyclonal Antibody, Unconjugated (TMAB-01994) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (ZDHHC6) Polyclonal Antibody, Unconjugated (TMAB-01994) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ZDHHC6

Human

ZDHHC6

Palmitoyltransferase ZDHHC6

Palmitoylates calnexin (CALX), which is required for its association with the ribosome-translocon complex and efficient folding of glycosylated proteins.