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Anti-ZCCHC4 Polyclonal Antibody is a Rabbit antibody targeting ZCCHC4. Anti-ZCCHC4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
| Description | Anti-ZCCHC4 Polyclonal Antibody is a Rabbit antibody targeting ZCCHC4. Anti-ZCCHC4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZCCHC4) Polyclonal Antibody, Unconjugated (TMAB-14267) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: JurkaT (Human) Cell Lysate at 30 μg Raji (Human) Cell Lysate at 30 μg Primary: Anti-ZCCHC4 (TMAB-14267) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | ZCCHC4 is a 513 amino acid protein that contains one CCHC-type zinc finger and one DHHC-type zinc finger and is thought to function as a methyltransferase. The gene encoding ZCCHC4 maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| Immunogen | KLH conjugated synthetic peptide: human ZCCHC4 |
| Antigen Species | Human |
| Gene Name | ZCCHC4 |
| Gene ID | |
| Protein Name | rRNA N6-adenosine-methyltransferase ZCCHC4 |
| Uniprot ID | |
| Function | May be a methyltransferase. |
| Molecular Weight | Theoretical: 59 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
| Size | Quantity | Unit Price | Amount | Operation |
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