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Anti-ZAP70 Antibody (2J161) (Synonyms: ZAP70, Tyrosine-protein kinase ZAP-70, Syk-related tyrosine kinase, SR...)
Catalog No. TMAC-04315 Copy Product Info
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Anti-ZAP70 Antibody (2J161) is a Rabbit antibody targeting ZAP70. Anti-ZAP70 Antibody (2J161) can be used in FCM,ICC,IHC,IP,WB.
Anti-ZAP70 Antibody (2J161)
Copy Product Info🥰Excellent
Catalog No. TMAC-04315
Synonyms ZAP70, Tyrosine-protein kinase ZAP-70, Syk-related tyrosine kinase, SRK, 70 kDa zeta-chain associated protein
Anti-ZAP70 Antibody (2J161) is a Rabbit antibody targeting ZAP70. Anti-ZAP70 Antibody (2J161) can be used in FCM,ICC,IHC,IP,WB.
Anti-ZAP70 Antibody
(2J161)
(2J161)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $496 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Related Conjugates and Formulations
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| Description | Anti-ZAP70 Antibody (2J161) is a Rabbit antibody targeting ZAP70. Anti-ZAP70 Antibody (2J161) can be used in FCM,ICC,IHC,IP,WB. |
| Synonyms | ZAP70, Tyrosine-protein kinase ZAP-70, Syk-related tyrosine kinase, SRK, 70 kDa zeta-chain associated protein |
| Ig Type | IgG |
| Clone | 2J161 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ZAP70 antibody. Counter stained with hematoxylin. 4. ICC staining ZAP70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining ZAP70 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Jurkat cells with ZAP70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.       |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and e chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 70 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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