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Anti-YKL-40/CHI3L1 Polyclonal Antibody 2 is a Rabbit antibody targeting YKL-40/CHI3L1. Anti-YKL-40/CHI3L1 Polyclonal Antibody 2 can be used in WB,ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB,ICC/IF Reactivity: Human,Mouse,Rat |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (TMAB-00426) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Protein: Liver (Rat) lysate at 30 μg; Colon carcinoma (Human) lysates at 30 μg; Primary: Anti-CGP-39/CHI3L1/YKL-40 (TMAB-00426) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000; Predicted band size: 42 kDa Observed band size: 39 kDa 3. Sample: Lane 1: Mouse Lung tissue lysates Lane 2: Rat Lung tissue lysates Primary: Anti-CHI3L1 (TMAB-00426) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa |
Application | WB,ICC/IF |
Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted, extracellular space. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009] |
Immunogen | KLH conjugated synthetic peptide: rat CHI3L1 |
Antigen Species | Rat |
Gene ID | 89824 |
Uniprot ID | |
Biology Area | Other ECM Proteins |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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