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Anti-YKL-40/CHI3L1 Polyclonal Antibody 2

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Catalog No. TMAB-00426

Anti-YKL-40/CHI3L1 Polyclonal Antibody 2 is a Rabbit antibody targeting YKL-40/CHI3L1. Anti-YKL-40/CHI3L1 Polyclonal Antibody 2 can be used in WB,ICC/IF.

Anti-YKL-40/CHI3L1 Polyclonal Antibody 2

Anti-YKL-40/CHI3L1 Polyclonal Antibody 2

😃Good
Catalog No. TMAB-00426
Anti-YKL-40/CHI3L1 Polyclonal Antibody 2 is a Rabbit antibody targeting YKL-40/CHI3L1. Anti-YKL-40/CHI3L1 Polyclonal Antibody 2 can be used in WB,ICC/IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB,ICC/IF

Reactivity: Human,Mouse,Rat
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (TMAB-00426) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Protein:
Liver (Rat) lysate at 30 μg;
Colon carcinoma (Human) lysates at 30 μg;
Primary: Anti-CGP-39/CHI3L1/YKL-40 (TMAB-00426) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000;
Predicted band size: 42 kDa
Observed band size: 39 kDa
3. Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Rat Lung tissue lysates
Primary: Anti-CHI3L1 (TMAB-00426) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Application
WB,ICC/IF
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:100
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundChitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: rat CHI3L1
Antigen Species
Rat
Gene ID
89824
Uniprot ID
Biology Area
Other ECM Proteins
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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