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Anti-YB1 Antibody (1K676) (Synonyms: ybx1, YBX 1, Y-box-binding protein 1, YBOX1_HUMAN, Y-box transcription ...)

Catalog No. TMAC-04310 Copy Product Info
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Anti-YB1 Antibody (1K676) is a Rabbit antibody targeting YB1. Anti-YB1 Antibody (1K676) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-YB1 Antibody (1K676)

Copy Product Info
🥰Excellent
Catalog No. TMAC-04310
Synonyms ybx1, YBX 1, Y-box-binding protein 1, YBOX1_HUMAN, Y-box transcription factor, Y-box binding protein 1, YB 1, Q15905, p50, Nuclease-sensitive element-binding protein 1, Nuclease sensitive element binding protein 1, NSEP1, NSEP 1, MGC117250, MGC110976, MGC104858, MDR NF1, Enhancer factor I subunit A, EFI-A, DNA-binding protein B, DNA binding protein B, DBPB, CSDB, CSDA2, CCAAT-binding transcription factor I subunit A, CCAAT binding transcription factor I subunit A, CBF-A, BP 8

Anti-YB1 Antibody (1K676) is a Rabbit antibody targeting YB1. Anti-YB1 Antibody (1K676) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-YB1 Antibody
(1K676)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4967-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-YB1 Antibody (1K676) is a Rabbit antibody targeting YB1. Anti-YB1 Antibody (1K676) can be used in FCM,ICC/IF,IHC,IP,WB.
Synonymsybx1, YBX 1, Y-box-binding protein 1, YBOX1_HUMAN, Y-box transcription factor, Y-box binding protein 1, YB 1, Q15905, p50, Nuclease-sensitive element-binding protein 1, Nuclease sensitive element binding protein 1, NSEP1, NSEP 1, MGC117250, MGC110976, MGC104858, MDR NF1, Enhancer factor I subunit A, EFI-A, DNA-binding protein B, DNA binding protein B, DBPB, CSDB, CSDA2, CCAAT-binding transcription factor I subunit A, CCAAT binding transcription factor I subunit A, CBF-A, BP 8
Ig Type
IgG
Clone
1K676
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-YB1 antibody. Counter stained with hematoxylin.
6. ICC staining YB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining YB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining YB1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundY-box binding protein YB-1 (also known as CCAAT-binding transcription factor, enhancer factor I subunit A and DNA-binding protein B) belongs to a family of multifunctional proteins which regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing Arginine clusters and aromatic residues. YB-1 plays a role in cell proliferation as an activator of growth-associated gene expression. YB-1 is also a repressor of the cell death-associated gene FAS. YB-1 may play an important role in controlling cell survival by regulating the expression of cell growth-associated and death-associated genes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 50 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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