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Anti-YB1 Antibody (1K676)
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Catalog No. TMAC-04310
Alias ybx1, YBX 1, Y-box-binding protein 1, YBOX1_HUMAN, Y-box transcription factor, Y-box binding protein 1, YB 1, Q15905, p50, Nuclease-sensitive element-binding protein 1, Nuclease sensitive element binding protein 1, NSEP1, NSEP 1, MGC117250, MGC110976, MGC104858, MDR NF1, Enhancer factor I subunit A, EFI-A, DNA-binding protein B, DNA binding protein B, DBPB, CSDB, CSDA2, CCAAT-binding transcription factor I subunit A, CCAAT binding transcription factor I subunit A, CBF-A, BP 8
Anti-YB1 Antibody (1K676) is a Rabbit antibody targeting YB1. Anti-YB1 Antibody (1K676) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-YB1 Antibody (1K676)
Anti-YB1 Antibody (1K676)
😃Good
Catalog No. TMAC-04310Alias ybx1, YBX 1, Y-box-binding protein 1, YBOX1_HUMAN, Y-box transcription factor, Y-box binding protein 1, YB 1, Q15905, p50, Nuclease-sensitive element-binding protein 1, Nuclease sensitive element binding protein 1, NSEP1, NSEP 1, MGC117250, MGC110976, MGC104858, MDR NF1, Enhancer factor I subunit A, EFI-A, DNA-binding protein B, DNA binding protein B, DBPB, CSDB, CSDA2, CCAAT-binding transcription factor I subunit A, CCAAT binding transcription factor I subunit A, CBF-A, BP 8
Anti-YB1 Antibody (1K676) is a Rabbit antibody targeting YB1. Anti-YB1 Antibody (1K676) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-YB1 Antibody (1K676) is a Rabbit antibody targeting YB1. Anti-YB1 Antibody (1K676) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | ybx1, YBX 1, Y-box-binding protein 1, YBOX1_HUMAN, Y-box transcription factor, Y-box binding protein 1, YB 1, Q15905, p50, Nuclease-sensitive element-binding protein 1, Nuclease sensitive element binding protein 1, NSEP1, NSEP 1, MGC117250, MGC110976, MGC104858, MDR NF1, Enhancer factor I subunit A, EFI-A, DNA-binding protein B, DNA binding protein B, DBPB, CSDB, CSDA2, CCAAT-binding transcription factor I subunit A, CCAAT binding transcription factor I subunit A, CBF-A, BP 8 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YB1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-YB1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-YB1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-YB1 antibody. Counter stained with hematoxylin. 6. ICC staining YB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining YB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining YB1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Y-box binding protein YB-1 (also known as CCAAT-binding transcription factor, enhancer factor I subunit A and DNA-binding protein B) belongs to a family of multifunctional proteins which regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing Arginine clusters and aromatic residues. YB-1 plays a role in cell proliferation as an activator of growth-associated gene expression. YB-1 is also a repressor of the cell death-associated gene FAS. YB-1 may play an important role in controlling cell survival by regulating the expression of cell growth-associated and death-associated genes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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