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Anti-Wnt8A Polyclonal Antibody

Catalog No. TMAB-14197 Copy Product Info
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Anti-Wnt8A Polyclonal Antibody is a Rabbit antibody targeting Wnt8A. Anti-Wnt8A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Wnt8A Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-14197

Anti-Wnt8A Polyclonal Antibody is a Rabbit antibody targeting Wnt8A. Anti-Wnt8A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Wnt8A Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Wnt8A Polyclonal Antibody is a Rabbit antibody targeting Wnt8A. Anti-Wnt8A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Horse,Sheep)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Wnt8A Polyclonal Antibody, Unconjugated (TMAB-14197) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-Wnt8A (TMAB-14197) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 48 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space, extracellular matrix.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. It encodes a protein which shows 81% amino acid identity to the mouse Wnt8A protein.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Wnt8A
Antigen Species
Human
Gene Name
WNT8A
Gene ID
Protein Name
Protein Wnt-8a
Uniprot ID
Function
Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Chemical Properties
Molecular WeightTheoretical: 36 kDa. Actual: 48 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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