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Anti-Wnt6 Polyclonal Antibody
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Catalog No. TMAB-14195 Copy Product Info
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Anti-Wnt6 Polyclonal Antibody is a Rabbit antibody targeting Wnt6. Anti-Wnt6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Wnt6 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $222 | 7-10 days | 7-10 days | |
| 100 µL | $371 | 7-10 days | 7-10 days | |
| 200 µL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Wnt6 Polyclonal Antibody is a Rabbit antibody targeting Wnt6. Anti-Wnt6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Wnt6 Polyclonal Antibody, Unconjugated (TMAB-14195) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: MKN45 (Human) Cell Lysate at 30 μg Primary: Anti-Wnt6 (TMAB-14195) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 42 kD 3. Sample: NIH/3T3 (Mouse) Cell Lysate at 30 μg Primary: Anti-Wnt6 (TMAB-14195) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 48 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Wnt6 is a member of the WNT protein family. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt6 is overexpressed in cervical and breast cell carcinomas and is co-expressed with Wnt10a in colorectal cancer cell lines. |
| Immunogen | KLH conjugated synthetic peptide: human Wnt6 |
| Antigen Species | Human |
| Gene Name | WNT6 |
| Gene ID | |
| Protein Name | Protein Wnt-6 |
| Uniprot ID |
| Molecular Weight | Theoretical: 38 kDa. Actual: 42-48 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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