Anti-WNT2B Polyclonal Antibody is a Rabbit antibody targeting WNT2B. Anti-WNT2B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Horse,Rabbit)

1. Protein:
lung (mouse) lysates at 30 μg;
liver (mouse) lysates at 30 μg;
Primary: Anti-WNT2b (TMAB-14190) at 1: 200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000;
ECL excitated the fluorescence;
Predicted band size: 4 4 kD
Observed band size: 48 kD
2. Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WNT2B) Polyclonal Antibody, Unconjugated (TMAB-14190) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Heart tissue lysates
Primary: Anti-WNT2B (TMAB-14190) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 47 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human WNT2B

Human

WNT2B

Protein Wnt-2b

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.