Anti-WNT10B Antibody (4Z727) is a Mouse antibody targeting WNT10B. Anti-WNT10B Antibody (4Z727) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG1, Kappa

4Z727

Human,Rat (predicted:Mouse)

1. Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-14184
2. Tissue: Human liver cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-14184
3. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:10000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: HeLa, 2: SH-SY5Y, 3: C2C12
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 43 kDa
Observed MW: 43 kDa
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WNT10B) Monoclonal Antibody, Unconjugated (TMAB-14184) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human WNT10B

Human

WNT10B

Protein Wnt-10b

Secreted, extracellular space, extracellular matrix.