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Anti-WISP2/CCN5 Polyclonal Antibody
😃Good
Catalog No. TMAB-01980
Alias WNT1-inducible-signaling pathway protein 2 (WISP-2), Wisp2, Ctgfl, Connective tissue growth factor-like protein (CTGF-L), Ccn5, CCN family member 5
Anti-WISP2/CCN5 Polyclonal Antibody is a Rabbit antibody targeting WISP2/CCN5. Anti-WISP2/CCN5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-WISP2/CCN5 Polyclonal Antibody
Anti-WISP2/CCN5 Polyclonal Antibody
😃Good
Catalog No. TMAB-01980Alias WNT1-inducible-signaling pathway protein 2 (WISP-2), Wisp2, Ctgfl, Connective tissue growth factor-like protein (CTGF-L), Ccn5, CCN family member 5
Anti-WISP2/CCN5 Polyclonal Antibody is a Rabbit antibody targeting WISP2/CCN5. Anti-WISP2/CCN5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-WISP2/CCN5 Polyclonal Antibody is a Rabbit antibody targeting WISP2/CCN5. Anti-WISP2/CCN5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | WNT1-inducible-signaling pathway protein 2 (WISP-2), Wisp2, Ctgfl, Connective tissue growth factor-like protein (CTGF-L), Ccn5, CCN family member 5 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WISP2) Polyclonal Antibody, Unconjugated (TMAB-01980) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: A431 Cell (Human) Lysate at 40 μg Primary: Anti-WISP2 (TMAB-01980) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa 3. Sample: A549 Cell (Human) Lysate at 40 μg Primary: Anti-WISP2 (TMAB-01980) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | WISP2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. WISP2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. |
| Immunogen | KLH conjugated synthetic peptide: human CTGFL |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 24 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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