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Anti-WISP2/CCN5 Polyclonal Antibody

😃Good
Catalog No. TMAB-01980
Alias WNT1-inducible-signaling pathway protein 2 (WISP-2), Wisp2, Ctgfl, Connective tissue growth factor-like protein (CTGF-L), Ccn5, CCN family member 5

Anti-WISP2/CCN5 Polyclonal Antibody is a Rabbit antibody targeting WISP2/CCN5. Anti-WISP2/CCN5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-WISP2/CCN5 Polyclonal Antibody

Anti-WISP2/CCN5 Polyclonal Antibody

😃Good
Catalog No. TMAB-01980Alias WNT1-inducible-signaling pathway protein 2 (WISP-2), Wisp2, Ctgfl, Connective tissue growth factor-like protein (CTGF-L), Ccn5, CCN family member 5
Anti-WISP2/CCN5 Polyclonal Antibody is a Rabbit antibody targeting WISP2/CCN5. Anti-WISP2/CCN5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-WISP2/CCN5 Polyclonal Antibody is a Rabbit antibody targeting WISP2/CCN5. Anti-WISP2/CCN5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasWNT1-inducible-signaling pathway protein 2 (WISP-2), Wisp2, Ctgfl, Connective tissue growth factor-like protein (CTGF-L), Ccn5, CCN family member 5
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WISP2) Polyclonal Antibody, Unconjugated (TMAB-01980) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample: A431 Cell (Human) Lysate at 40 μg
Primary: Anti-WISP2 (TMAB-01980) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
3. Sample: A549 Cell (Human) Lysate at 40 μg
Primary: Anti-WISP2 (TMAB-01980) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundWISP2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. WISP2 lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CTGFL
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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