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Anti-Wilms tumor/WT1 Polyclonal Antibody

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Catalog No. TMAB-01979

Anti-Wilms tumor/WT1 Polyclonal Antibody is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Wilms tumor/WT1 Polyclonal Antibody

Anti-Wilms tumor/WT1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01979
Anti-Wilms tumor/WT1 Polyclonal Antibody is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Wilms tumor/WT1 Polyclonal Antibody is a Rabbit antibody targeting Wilms tumor/WT1. Anti-Wilms tumor/WT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Wilms Tumor Protein) Polyclonal Antibody, Unconjugated (TMAB-01979) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nucleus staining.
2. Sample: TM4 Cell (Mouse) Lysate at 40 μg
Primary: Anti-Wilms Tumor Protein (TMAB-01979) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 48 kDa
3. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-WT-1/Wilms Tumor Protein Polyclonal Antibody, Unconjugated (TMAB-01979) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-Wilms Tumor Protein antibody (TMAB-01979)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Wilms Tumor Protein) Polyclonal Antibody, Unconjugated (TMAB-01979) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Ovary tissue lysates
Lane 3: Mouse Liver tissue lysates
Lane 4: Human HL-60 cell lysates
Primary: Anti-WT1 (TMAB-01979) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 50 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Nucleus, nucleolus. Cytoplasm. Note=Shuttles between nucleus and cytoplasm.Isoform 1: Nucleus speckle.Isoform 4: Nucleus, nucleoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundTranscription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human WT1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Tumor biomarkers,Kidney development,Other factors,Tumor Associated
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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