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Anti-WFDC2 Antibody (4W741)

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Catalog No. TMAC-01793
Alias WAP four-disulfide core domain protein 2, Putative protease inhibitor WAP5, Major epididymis-specific protein E4, Epididymal secretory protein E4

Anti-WFDC2 Antibody (4W741) is a Rabbit antibody targeting WFDC2. Anti-WFDC2 Antibody (4W741) can be used in WB,ICC/IF,IHC,IP,FCM.

Anti-WFDC2 Antibody (4W741)

Anti-WFDC2 Antibody (4W741)

😃Good
Catalog No. TMAC-01793Alias WAP four-disulfide core domain protein 2, Putative protease inhibitor WAP5, Major epididymis-specific protein E4, Epididymal secretory protein E4
Anti-WFDC2 Antibody (4W741) is a Rabbit antibody targeting WFDC2. Anti-WFDC2 Antibody (4W741) can be used in WB,ICC/IF,IHC,IP,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-WFDC2 Antibody (4W741) is a Rabbit antibody targeting WFDC2. Anti-WFDC2 Antibody (4W741) can be used in WB,ICC/IF,IHC,IP,FCM.
SynonymsWAP four-disulfide core domain protein 2, Putative protease inhibitor WAP5, Major epididymis-specific protein E4, Epididymal secretory protein E4
Ig Type
IgG
Clone
4W741
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control: Lane 1: SW480, Lane 2: SKOV-3.
2. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.
3. ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining HE4 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of SW480 cells with HE4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundHE4 (whey acidic protein (WAP)-type four-disulfide core-2, WFDC2) is a small secretory protein that may influence sperm maturation. HE4 gene expression is high in pulmonary epithelial cells and in some ovarian cancers. HE4 protein has a WAP motif that contains eight cysteines forming four disulfide bonds at the core of the protein. The WAP motif functions as a protease inhibitor in many of the family members that contain them.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 23 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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