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Anti-WEE1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01978
Alias WEE1hu, Wee1A kinase, WEE1A, Wee1+S. pombe homolog, Wee1+homolog, WEE1 tyrosine kinase isoform 1, Wee1 tyrosine kinase, Wee1 like protein kinase, WEE1 homolog S. pombe, Wee1 homolog, WEE-1, MGC105683, FLJ16446, EC 2.7.10.2, DKFZp686I18166
Anti-WEE1 Polyclonal Antibody is a Rabbit antibody targeting WEE1. Anti-WEE1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-WEE1 Polyclonal Antibody
Anti-WEE1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01978Alias WEE1hu, Wee1A kinase, WEE1A, Wee1+S. pombe homolog, Wee1+homolog, WEE1 tyrosine kinase isoform 1, Wee1 tyrosine kinase, Wee1 like protein kinase, WEE1 homolog S. pombe, Wee1 homolog, WEE-1, MGC105683, FLJ16446, EC 2.7.10.2, DKFZp686I18166
Anti-WEE1 Polyclonal Antibody is a Rabbit antibody targeting WEE1. Anti-WEE1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-WEE1 Polyclonal Antibody is a Rabbit antibody targeting WEE1. Anti-WEE1 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | WEE1hu, Wee1A kinase, WEE1A, Wee1+S. pombe homolog, Wee1+homolog, WEE1 tyrosine kinase isoform 1, Wee1 tyrosine kinase, Wee1 like protein kinase, WEE1 homolog S. pombe, Wee1 homolog, WEE-1, MGC105683, FLJ16446, EC 2.7.10.2, DKFZp686I18166 |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. Sample: Heart (Rat) Lysate at 40 μg Primary: Anti-WEE1 (TMAB-01978) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WEE1) Polyclonal Antibody, Unconjugated (TMAB-01978) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.   |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. |
| Immunogen | KLH conjugated synthetic peptide: human WEE1 |
| Antigen Species | Human |
| Gene Name | WEE1 |
| Gene ID | |
| Protein Name | Wee1-like protein kinase |
| Uniprot ID | |
| Biology Area | Cdk Regulators,Cdk Regulators |
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation |
| Molecular Weight | Theoretical: 72 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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