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Anti-WEE1 Polyclonal Antibody is a Rabbit antibody targeting WEE1. Anti-WEE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-WEE1 Polyclonal Antibody is a Rabbit antibody targeting WEE1. Anti-WEE1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | WEE1hu, Wee1A kinase, WEE1A, Wee1+S. pombe homolog, Wee1+homolog, WEE1 tyrosine kinase isoform 1, Wee1 tyrosine kinase, Wee1 like protein kinase, WEE1 homolog S. pombe, Wee1 homolog, WEE-1, MGC105683, FLJ16446, EC 2.7.10.2, DKFZp686I18166 |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse) |
Verified Activity | 1. Sample: Heart (Rat) Lysate at 40 μg Primary: Anti-WEE1 (TMAB-01978) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WEE1) Polyclonal Antibody, Unconjugated (TMAB-01978) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. |
Immunogen | KLH conjugated synthetic peptide: human WEE1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Cdk Regulators,Cdk Regulators |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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