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Anti-WDR26 Polyclonal Antibody

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Catalog No. TMAB-01977
Alias WDR26, WD repeat-containing protein 26, WD repeat domain 26, PRO0852, myocardial ischemic preconditioning upregulated protein 2, Myocardial ischemic preconditioning up regulated protein 2, MIP2, FLJ21016, CUL4 and DDB1 associated WDR protein 2, CDW2

Anti-WDR26 Polyclonal Antibody is a Rabbit antibody targeting WDR26. Anti-WDR26 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-WDR26 Polyclonal Antibody

Anti-WDR26 Polyclonal Antibody

😃Good
Catalog No. TMAB-01977Alias WDR26, WD repeat-containing protein 26, WD repeat domain 26, PRO0852, myocardial ischemic preconditioning upregulated protein 2, Myocardial ischemic preconditioning up regulated protein 2, MIP2, FLJ21016, CUL4 and DDB1 associated WDR protein 2, CDW2
Anti-WDR26 Polyclonal Antibody is a Rabbit antibody targeting WDR26. Anti-WDR26 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-WDR26 Polyclonal Antibody is a Rabbit antibody targeting WDR26. Anti-WDR26 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
AliasWDR26, WD repeat-containing protein 26, WD repeat domain 26, PRO0852, myocardial ischemic preconditioning upregulated protein 2, Myocardial ischemic preconditioning up regulated protein 2, MIP2, FLJ21016, CUL4 and DDB1 associated WDR protein 2, CDW2
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse)
Verified Activity
1. Sample:
Placenta (Mouse) Lysate at 40 μg
Primary: Anti-WDR26 (TMAB-01977) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 70 kDa
2. Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated (TMAB-01977) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
4. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated (TMAB-01977) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-WDR26 antibody (TMAB-01977)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
6. Tissue/cell: MCF7; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (WDR26) Polyclonal Antibody, Unconjugated (TMAB-01977) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1µg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human WDR26
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Oxidative Stress,Oxidative stress,MAPK Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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