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Anti-WDFY3 Polyclonal Antibody

Catalog No. TMAB-14164 Copy Product Info
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Anti-WDFY3 Polyclonal Antibody is a Rabbit antibody targeting WDFY3. Anti-WDFY3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-WDFY3 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-14164

Anti-WDFY3 Polyclonal Antibody is a Rabbit antibody targeting WDFY3. Anti-WDFY3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-WDFY3 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-WDFY3 Polyclonal Antibody is a Rabbit antibody targeting WDFY3. Anti-WDFY3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WDFY3) Polyclonal Antibody, Unconjugated (TMAB-14164) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (WDFY3) Polyclonal Antibody, Unconjugated (TMAB-14164) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
Tissue SpecificityUbiquitous.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundWDFY3 was identified in a screen for phosphatidylinositol 3 phosphate binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human WDFY3
Antigen Species
Human
Gene Name
WDFY3
Gene ID
Protein Name
WD repeat and FYVE domain-containing protein 3
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 395 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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