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Anti-VWF Antibody (9I145)
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Catalog No. TMAB-14151 Copy Product Info
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Anti-VWF Antibody (9I145) is a Rabbit antibody targeting VWF. Anti-VWF Antibody (9I145) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-VWF Antibody
(9I145)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 25 µL | $151 | 7-10 days | 7-10 days | |
| 50 µL | $264 | 7-10 days | 7-10 days | |
| 100 µL | $471 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-VWF Antibody (9I145) is a Rabbit antibody targeting VWF. Anti-VWF Antibody (9I145) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 9I145 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VWF Monoclonal Antibody, Unconjugated (TMAB-14151) at 1:1000 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:800-2000; IHC-Fr: 1:800-2000; IF: 1:800-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules. |
| Tissue Specificity | Plasma. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300. |
| Concentration | 1mg/ml |
| Research Background | Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.). |
| Immunogen | Recombinant Protein: human VWF protein |
| Antigen Species | Human |
| Gene Name | VWF |
| Gene ID | |
| Protein Name | von Willebrand factor |
| Uniprot ID | |
| Function | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. |
| Molecular Weight | Theoretical: 309 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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