Anti-VPS28 Antibody (1W518) is a Rabbit antibody targeting VPS28. Anti-VPS28 Antibody (1W518) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

1W518

Human,Mouse,Rat

1. Western blot analysis of Jurkat cell lysate. Using VPS28 (TMAB-14135) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-VPS28 antibody (TMAB-14135, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human VPS28

Human

VPS28

Vacuolar protein sorting-associated protein 28 homolog

Component of the ESCRT-I complex, a regulator of vesicular trafficking process.