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Anti-VPS18 Antibody (9Y368)
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Catalog No. TMAB-14129
Anti-VPS18 Antibody (9Y368) is a Mouse antibody targeting VPS18. Anti-VPS18 Antibody (9Y368) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-VPS18 Antibody (9Y368)
Anti-VPS18 Antibody (9Y368)
Copy Product Info😃Good
Catalog No. TMAB-14129
Anti-VPS18 Antibody (9Y368) is a Mouse antibody targeting VPS18. Anti-VPS18 Antibody (9Y368) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-VPS18 Antibody (9Y368) is a Mouse antibody targeting VPS18. Anti-VPS18 Antibody (9Y368) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | Ig2A/Kappa |
| Clone | 9Y368 |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. Cell line: HeLa Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for TMAB-14129 2. Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HeLa, 293, A20, BRL Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 100 kDa Observed MW: 100 kDa 3. Tissue: Rat heart Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal forTMAB-14129 4. Tissue: Human liver cancer Section type: Formalin fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit (Mouse) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-14129 |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes. |
| Tissue Specificity | Ubiquitous. Expression was highest in heart and low in lung. |
| Construction | Recombinant Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. |
| Gene Name | VPS18 |
| Gene ID | |
| Protein Name | Vacuolar protein sorting-associated protein 18 homolog |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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