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Anti-VPS18 Antibody (3X723)

Catalog No. TMAB-14130 Copy Product Info
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Anti-VPS18 Antibody (3X723) is a Rabbit antibody targeting VPS18. Anti-VPS18 Antibody (3X723) can be used in WB,ICC/IF,FCM.

Anti-VPS18 Antibody (3X723)

Copy Product Info
🥰Excellent
Catalog No. TMAB-14130

Anti-VPS18 Antibody (3X723) is a Rabbit antibody targeting VPS18. Anti-VPS18 Antibody (3X723) can be used in WB,ICC/IF,FCM.

Anti-VPS18 Antibody
(3X723)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1517-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4707-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-VPS18 Antibody (3X723) is a Rabbit antibody targeting VPS18. Anti-VPS18 Antibody (3X723) can be used in WB,ICC/IF,FCM.
Ig Type
IgG
Clone
3X723
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of K562 cell lysate. Using VPS18 (TMAB-14130) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The K562 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-VPS18 antibody (TMAB-14130, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
WB: 1:1000-2000; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationLate endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
Tissue SpecificityUbiquitous. Expression was highest in heart and low in lung.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundPlays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion.
Antigen Details
Immunogen
A synthesized peptide: human VPS18
Antigen Species
Human
Gene Name
VPS18
Gene ID
Protein Name
Vacuolar protein sorting-associated protein 18 homolog
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 110 kDa. Actual: 100 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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