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Anti-VPS11 Antibody (9C164)

Catalog No. TMAB-14127 Copy Product Info
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Anti-VPS11 Antibody (9C164) is a Rabbit antibody targeting VPS11. Anti-VPS11 Antibody (9C164) can be used in WB,IHC-P,IHC-Fr,FCM,IF.

Anti-VPS11 Antibody (9C164)

Copy Product Info
🥰Excellent
Catalog No. TMAB-14127

Anti-VPS11 Antibody (9C164) is a Rabbit antibody targeting VPS11. Anti-VPS11 Antibody (9C164) can be used in WB,IHC-P,IHC-Fr,FCM,IF.

Anti-VPS11 Antibody
(9C164)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1507-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4737-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-VPS11 Antibody (9C164) is a Rabbit antibody targeting VPS11. Anti-VPS11 Antibody (9C164) can be used in WB,IHC-P,IHC-Fr,FCM,IF.
Ig Type
IgG
Clone
9C164
Reactivity
Human (predicted:Mouse)
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with VPS11 monoclonal antibody, unconjugated (TMAB-14127) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The K562 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-VPS11 antibody (TMAB-14127, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; FCM: 1:50-100; IF: 1:100-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationLate endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
Tissue SpecificityUbiquitous. Expression was highest in heart and low in lung.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundPlays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion.
Antigen Details
Immunogen
A synthesized peptide: human VPS11
Antigen Species
Human
Gene Name
VPS11
Gene ID
Protein Name
Vacuolar protein sorting-associated protein 11 homolog
Uniprot ID
Function
May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Chemical Properties
Molecular WeightTheoretical: 108 kDa. Actual: 108 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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