Shopping Cart
- Remove All
- Your shopping cart is currently empty
Anti-Von Willebrand Factor/vWF Polyclonal Antibody is a Rabbit antibody targeting Von Willebrand Factor/vWF. Anti-Von Willebrand Factor/vWF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Von Willebrand Factor/vWF Polyclonal Antibody is a Rabbit antibody targeting Von Willebrand Factor/vWF. Anti-Von Willebrand Factor/vWF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
Alias | VWF, VWD, F8VWF |
Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit) |
Verified Activity | 1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-VWF Polyclonal Antibody, Unconjugated (TMAB-01972) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-VWF Polyclonal Antibody, Unconjugated (TMAB-01972) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VWF) Polyclonal Antibody, Unconjugated (TMAB-01972) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
Application | IHC-P,IHC-Fr,IF |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.). |
Immunogen | KLH conjugated synthetic peptide: human von Willebrand antigen 2 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Angiogenic growth factors,Endothelial Cell Markers,Extrinsic,Regulatory,Platelets,Serum Proteins,Endothelial Markers |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.