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Anti-Von Willebrand Factor/vWF Polyclonal Antibody

😃Good
Catalog No. TMAB-01972
Alias VWF, VWD, F8VWF

Anti-Von Willebrand Factor/vWF Polyclonal Antibody is a Rabbit antibody targeting Von Willebrand Factor/vWF. Anti-Von Willebrand Factor/vWF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Von Willebrand Factor/vWF Polyclonal Antibody

Anti-Von Willebrand Factor/vWF Polyclonal Antibody

😃Good
Catalog No. TMAB-01972Alias VWF, VWD, F8VWF
Anti-Von Willebrand Factor/vWF Polyclonal Antibody is a Rabbit antibody targeting Von Willebrand Factor/vWF. Anti-Von Willebrand Factor/vWF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Von Willebrand Factor/vWF Polyclonal Antibody is a Rabbit antibody targeting Von Willebrand Factor/vWF. Anti-Von Willebrand Factor/vWF Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
AliasVWF, VWD, F8VWF
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit)
Verified Activity
1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VWF Polyclonal Antibody, Unconjugated (TMAB-01972) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VWF Polyclonal Antibody, Unconjugated (TMAB-01972) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VWF) Polyclonal Antibody, Unconjugated (TMAB-01972) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundVon Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human von Willebrand antigen 2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Angiogenic growth factors,Endothelial Cell Markers,Extrinsic,Regulatory,Platelets,Serum Proteins,Endothelial Markers
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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