Shopping Cart
- Remove All
- Your shopping cart is currently empty
Anti-Vimentin Antibody (5Y646) is a Rabbit antibody targeting Vimentin. Anti-Vimentin Antibody (5Y646) can be used in WB,IHC-P.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $93 | 7-10 days | |
100 μL | $268 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: WB, IHC-P Reactivity: Human |
Alias | vimentin, HEL113, CTRCT30 |
Ig Type | Rabbit IgG |
Clone | 5Y646 |
Reactivity | Human |
Verified Activity | 1. Anti-VIM rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: A549 Whole Cell Lysate. -Lane C: 293T Whole Cell Lysate. -Lane D: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:54 kDa. -Observed band size:55 kDa. 2. Immunochemical staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. 4. Immunochemical staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. |
Application | WB,IHC-P |
Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:1000-1:10000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Vimentin/VIM. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: center region of the Human Vimentin/VIM |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.