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Anti-Vimentin Antibody (5Y646)

😃Good
Catalog No. TMAY-00097
Alias vimentin, HEL113, CTRCT30

Anti-Vimentin Antibody (5Y646) is a Rabbit antibody targeting Vimentin. Anti-Vimentin Antibody (5Y646) can be used in WB,IHC-P.

Anti-Vimentin Antibody (5Y646)

Anti-Vimentin Antibody (5Y646)

😃Good
Catalog No. TMAY-00097Alias vimentin, HEL113, CTRCT30
Anti-Vimentin Antibody (5Y646) is a Rabbit antibody targeting Vimentin. Anti-Vimentin Antibody (5Y646) can be used in WB,IHC-P.
Pack SizePriceAvailabilityQuantity
20 μL$93 7-10 days
100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Monoclonal

Application: WB, IHC-P

Reactivity: Human
Aliasvimentin, HEL113, CTRCT30
Ig Type
Rabbit IgG
Clone
5Y646
Reactivity
Human
Verified Activity
1. Anti-VIM rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Hela Whole Cell Lysate.
-Lane B: A549 Whole Cell Lysate.
-Lane C: 293T Whole Cell Lysate.
-Lane D: Jurkat Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:54 kDa.
-Observed band size:55 kDa.
2. Immunochemical staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.
3. Immunochemical staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.
4. Immunochemical staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.
Application
WB,IHC-P
Recommended Dose
WB: 1:500-1:2000; IHC-P: 1:1000-1:10000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Vimentin/VIM.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundVimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: center region of the Human Vimentin/VIM
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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