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Anti-VGLL4 Polyclonal Antibody
😃Good
Catalog No. TMAB-01961
Anti-VGLL4 Polyclonal Antibody is a Rabbit antibody targeting VGLL4. Anti-VGLL4 Polyclonal Antibody can be used in WB.
Anti-VGLL4 Polyclonal Antibody
Anti-VGLL4 Polyclonal Antibody
😃Good
Catalog No. TMAB-01961
Anti-VGLL4 Polyclonal Antibody is a Rabbit antibody targeting VGLL4. Anti-VGLL4 Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-VGLL4 Polyclonal Antibody is a Rabbit antibody targeting VGLL4. Anti-VGLL4 Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Ovary (Mouse) Lysate at 40 μg Ovary (Rat) Lysate at 40 μg Primary: Anti-VGLL4 (TMAB-01961) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa 2. Sample: Lane 1: Mouse Stomach tissue lysates Lane 2: Mouse Lung tissue lysates Lane 3: Mouse Large intestine tissue lysates Primary: Anti-VGLL4 (TMAB-01961) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nuclear |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The transcriptional enhancer factor-1 (TEF-1) family of transcription factors regulate tissue-specific gene expression in muscle and placenta. The mechanism whereby TEF-1 confers tissue specificity depends largely on the interaction of TEF-1 with tissue-specific cofactors. Transcription cofactor Vgl-4 (vestigial-like protein 4) is a 290 amino acid nuclear protein that interacts with TEF-1 and MEF-2. Vgl-4 is the only member of the vestigial-like family that is expressed in heart. Overexpression of Vgl-4 in cardiac myocytes interferes with basal expression and ?-adrenergic receptor-dependent activation of a TEF-1 dependent skeletal ?actin promoter. This suggests that Vgl-4 counteracts ?-adrenergic activation of gene expression in cardiomyocytes. There are two isoforms of Vgl-4 that are produced as a result of alternative splicing events. |
| Immunogen | KLH conjugated synthetic peptide: human VGLL4 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Co-factors,Other factors |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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