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Anti-Vesicle docking protein p115 Polyclonal Antibody
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Catalog No. TMAB-14090
Anti-Vesicle docking protein p115 Polyclonal Antibody is a Rabbit antibody targeting Vesicle docking protein p115. Anti-Vesicle docking protein p115 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Vesicle docking protein p115 Polyclonal Antibody
Anti-Vesicle docking protein p115 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-14090
Anti-Vesicle docking protein p115 Polyclonal Antibody is a Rabbit antibody targeting Vesicle docking protein p115. Anti-Vesicle docking protein p115 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Vesicle docking protein p115 Polyclonal Antibody is a Rabbit antibody targeting Vesicle docking protein p115. Anti-Vesicle docking protein p115 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Pig,Cow,Horse,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Vesicle docking protein p115) Polyclonal Antibody, Unconjugated (TMAB-14090) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Sample: A431 (Human) Cell Lysate at 30 μg 293T (Human) Cell Lysate at 30 μg Primary: Anti-USO1 (TMAB-14090) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 120 kD 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Vesicle docking protein p115) Polyclonal Antibody, Unconjugated (TMAB-14090) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Primary: Anti-Vesicle docking protein p115 (TMAB-14090) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kDa Observed band size: 140 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm; cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | p115 (Vesicle docking protein p115) is a peripheral membrane protein that is located on the Golgi complex. p115 exists as a homodimer with two globular heads, an extended coiled-coil tail, followed by an acidic domain at the extreme C terminus. p115 is homologous to a yeast protein, Uso1p, which is required for ER to Golgi transport. p115 likely plays an important role in vesicle transportation from the ER to the cis-Golgi comparments. |
| Immunogen | KLH conjugated synthetic peptide: human Vesicle docking protein p115 |
| Antigen Species | Human |
| Gene Name | USO1 |
| Gene ID | |
| Protein Name | General vesicular transport factor p115 |
| Uniprot ID | |
| Function | General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. |
| Molecular Weight | Theoretical: 108 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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