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Anti-VDAC3 Polyclonal Antibody
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Catalog No. TMAB-01944
Alias voltage dependent anion channel 3, VDAC 3, Outer mitochondrial membrane protein porin 3, mVDAC3, hVDAC3
Anti-VDAC3 Polyclonal Antibody is a Rabbit antibody targeting VDAC3. Anti-VDAC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-VDAC3 Polyclonal Antibody
Anti-VDAC3 Polyclonal Antibody
😃Good
Catalog No. TMAB-01944Alias voltage dependent anion channel 3, VDAC 3, Outer mitochondrial membrane protein porin 3, mVDAC3, hVDAC3
Anti-VDAC3 Polyclonal Antibody is a Rabbit antibody targeting VDAC3. Anti-VDAC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-VDAC3 Polyclonal Antibody is a Rabbit antibody targeting VDAC3. Anti-VDAC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | voltage dependent anion channel 3, VDAC 3, Outer mitochondrial membrane protein porin 3, mVDAC3, hVDAC3 |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-VDAC3 Polyclonal Antibody, Unconjugated (TMAB-01944) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Large intestine (Rat) Lysate at 40 μg Primary: Anti-VDAC3 (TMAB-01944) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa 3. Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Spleen tissue lysates Lane 3: Rat Kidney tissue lysates Lane 4: Rat Spleen tissue lysates Primary: Anti-VDAC3 (TMAB-01944) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Mitochondrion outer membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a voltage-dependent anion channel (VDAC), and belongs to the mitochondrial porin family. VDACs are small, integral membrane proteins that traverse the outer mitochondrial membrane and conduct ATP and other small metabolites. They are known to bind several kinases of intermediary metabolism, thought to be involved in translocation of adenine nucleotides, and are hypothesized to form part of the mitochondrial permeability transition pore, which results in the release of cytochrome c at the onset of apoptotic cell death. Alternatively transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] |
| Immunogen | KLH conjugated synthetic peptide: human VDAC3 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 31 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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