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Anti-VDAC3 Polyclonal Antibody

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Catalog No. TMAB-01944
Alias voltage dependent anion channel 3, VDAC 3, Outer mitochondrial membrane protein porin 3, mVDAC3, hVDAC3

Anti-VDAC3 Polyclonal Antibody is a Rabbit antibody targeting VDAC3. Anti-VDAC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-VDAC3 Polyclonal Antibody

Anti-VDAC3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01944Alias voltage dependent anion channel 3, VDAC 3, Outer mitochondrial membrane protein porin 3, mVDAC3, hVDAC3
Anti-VDAC3 Polyclonal Antibody is a Rabbit antibody targeting VDAC3. Anti-VDAC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-VDAC3 Polyclonal Antibody is a Rabbit antibody targeting VDAC3. Anti-VDAC3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Aliasvoltage dependent anion channel 3, VDAC 3, Outer mitochondrial membrane protein porin 3, mVDAC3, hVDAC3
Reactivity
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VDAC3 Polyclonal Antibody, Unconjugated (TMAB-01944) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Large intestine (Rat) Lysate at 40 μg
Primary: Anti-VDAC3 (TMAB-01944) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
3. Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Rat Spleen tissue lysates
Primary: Anti-VDAC3 (TMAB-01944) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion outer membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a voltage-dependent anion channel (VDAC), and belongs to the mitochondrial porin family. VDACs are small, integral membrane proteins that traverse the outer mitochondrial membrane and conduct ATP and other small metabolites. They are known to bind several kinases of intermediary metabolism, thought to be involved in translocation of adenine nucleotides, and are hypothesized to form part of the mitochondrial permeability transition pore, which results in the release of cytochrome c at the onset of apoptotic cell death. Alternatively transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human VDAC3
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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