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Anti-VCP Antibody (3I403) (Synonyms: VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPas...)

Catalog No. TMAC-04229 Copy Product Info
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Anti-VCP Antibody (3I403) is a Rabbit antibody targeting VCP. Anti-VCP Antibody (3I403) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-VCP Antibody (3I403)

Copy Product Info
🥰Excellent
Catalog No. TMAC-04229
Synonyms VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit

Anti-VCP Antibody (3I403) is a Rabbit antibody targeting VCP. Anti-VCP Antibody (3I403) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-VCP Antibody
(3I403)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2977-10 days7-10 days
100 μL$4977-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-VCP Antibody (3I403) is a Rabbit antibody targeting VCP. Anti-VCP Antibody (3I403) can be used in FCM,ICC/IF,IHC,IP,WB.
SynonymsVCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit
Ig Type
IgG
Clone
3I403
Reactivity
Human,Mouse,Rat,zebrafish
Verified Activity
1. Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution. Positive control: Line 1: SH-SY5Y, Line 2: HepG2, Line 3: Hela.
2. Western blot analysis of VCP on hybrid fish (crucian-carp) heart tissue lysate using anti-VCP antibody at 1/500 dilution.
3. Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-VCP antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-VCP antibody. Counter stained with hematoxylin.
7. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VCP antibody. Counter stained with hematoxylin.
8. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VCP antibody. Counter stained with hematoxylin.
9. ICC staining VCP in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. ICC staining VCP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
11. ICC staining VCP in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
12. Flow cytometric analysis of HL-60 cells with VCP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundValosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-α as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 89 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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