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Anti-VCP Antibody (3I403)
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Catalog No. TMAC-04229
Alias VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit
Anti-VCP Antibody (3I403) is a Rabbit antibody targeting VCP. Anti-VCP Antibody (3I403) can be used in WB,ICC/IF,IHC,FCM,IP.
Anti-VCP Antibody (3I403)
Anti-VCP Antibody (3I403)
😃Good
Catalog No. TMAC-04229Alias VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit
Anti-VCP Antibody (3I403) is a Rabbit antibody targeting VCP. Anti-VCP Antibody (3I403) can be used in WB,ICC/IF,IHC,FCM,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-VCP Antibody (3I403) is a Rabbit antibody targeting VCP. Anti-VCP Antibody (3I403) can be used in WB,ICC/IF,IHC,FCM,IP. |
| Synonyms | VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,zebrafish |
| Verified Activity | 1. Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution. Positive control: Line 1: SH-SY5Y, Line 2: HepG2, Line 3: Hela. 2. Western blot analysis of VCP on hybrid fish (crucian-carp) heart tissue lysate using anti-VCP antibody at 1/500 dilution. 3. Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-VCP antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VCP antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-VCP antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-VCP antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VCP antibody. Counter stained with hematoxylin. 8. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VCP antibody. Counter stained with hematoxylin. 9. ICC staining VCP in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining VCP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 11. ICC staining VCP in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 12. Flow cytometric analysis of HL-60 cells with VCP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Valosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-α as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 89 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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