Anti-VCP Antibody (1O48) is a Mouse antibody targeting VCP. Anti-VCP Antibody (1O48) can be used in WB,ICC/IF.

IgG1, k

1O48

Human,Mouse,Rat

1. U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (VCP) monoclonal Antibody, Unconjugated (TMAB-14060) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes,Alexa Fluor® 555 conjugated with Phalloidin (red) was used to stain the cell Cytoplasmic actin.
2. Sample:
Lane 1: MCF-7 cell lysates
Lane 2: U251 cell lysates
Lane 3: NIH/3T3 cell lysates
Lane 4: C6 cell lysates
Lane 5: Mouse Liver tissue lysates
Primary: Anti-VCP (TMAB-14060) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 97 kD
Observed band size: 105 kD

WB: 1:500-2000; ICC/IF: 1:50-200

Monoclonal

Recombinant Protein: human VCP

Human

VCP

Transitional endoplasmic reticulum ATPase

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.