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Anti-VCAM-1 Antibody (5T238)

🥰Excellent
Catalog No. TMAY-03150

Anti-VCAM-1 Antibody (5T238) is a Rabbit antibody targeting VCAM-1. Anti-VCAM-1 Antibody (5T238) can be used in WB,FCM.

Anti-VCAM-1 Antibody (5T238)

Anti-VCAM-1 Antibody (5T238)

🥰Excellent
Catalog No. TMAY-03150
Anti-VCAM-1 Antibody (5T238) is a Rabbit antibody targeting VCAM-1. Anti-VCAM-1 Antibody (5T238) can be used in WB,FCM.
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Product Introduction

Bioactivity
Description
Anti-VCAM-1 Antibody (5T238) is a Rabbit antibody targeting VCAM-1. Anti-VCAM-1 Antibody (5T238) can be used in WB,FCM.
Ig Type
Monoclonal Rabbit IgG
Clone
5T238
Reactivity
Mouse
Verified Activity
1. Anti-VCAM1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: NIH 3T3 Whole Cell Lysate.

-Lane B: Mouse brain tissue Lysate.

-Lane C: Mouse kidney tissue lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size:81 kDa.

-Observed band size:100 kDa.

2. Flow cytometric analysis of Mouse VCAM1(CD106) expression on BABL/c bone marrow cells. Cells were stained with purified anti-Mouse VCAM1(CD106), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
WB,FCM
Recommended Dose
WB: 1:500-1:2000; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Mouse VCAM1/VCAM-1/CD106 (rM VCAM1/VCAM-1/CD106; TMPY-01830; NP_035823.3; Met1-Glu698).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundVascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC/PE
Antibody Types Available
3
Antigen Details
Immunogen
Recombinant Mouse VCAM1/VCAM-1/CD106 Protein (TMPY-01830)
Antigen Species
Mouse
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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