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Anti-VCAM-1 Antibody (5T238) is a Rabbit antibody targeting VCAM-1. Anti-VCAM-1 Antibody (5T238) can be used in WB,FCM.
Description | Anti-VCAM-1 Antibody (5T238) is a Rabbit antibody targeting VCAM-1. Anti-VCAM-1 Antibody (5T238) can be used in WB,FCM. |
Ig Type | Monoclonal Rabbit IgG |
Clone | 5T238 |
Reactivity | Mouse |
Verified Activity | 1. Anti-VCAM1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: NIH 3T3 Whole Cell Lysate. -Lane B: Mouse brain tissue Lysate. -Lane C: Mouse kidney tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:81 kDa. -Observed band size:100 kDa. 2. Flow cytometric analysis of Mouse VCAM1(CD106) expression on BABL/c bone marrow cells. Cells were stained with purified anti-Mouse VCAM1(CD106), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
Application | WB,FCM |
Recommended Dose | WB: 1:500-1:2000; FCM: 1:25-1:100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse VCAM1/VCAM-1/CD106 (rM VCAM1/VCAM-1/CD106; TMPY-01830; NP_035823.3; Met1-Glu698). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
Conjucates | Unconjugated |
Others Formats | FITC/PE |
Antibody Types Available | 3 |
Immunogen | Recombinant Mouse VCAM1/VCAM-1/CD106 Protein (TMPY-01830) |
Antigen Species | Mouse |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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