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Anti-VAMP3 Antibody (5C283)
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Catalog No. TMAC-00812
Alias vesicle-associated membrane protein 3, CEB
Anti-VAMP3 Antibody (5C283) is a Rabbit antibody targeting VAMP3. Anti-VAMP3 Antibody (5C283) can be used in WB,ICC,IF,IHC,FCM.
Anti-VAMP3 Antibody (5C283)
Anti-VAMP3 Antibody (5C283)
😃Good
Catalog No. TMAC-00812Alias vesicle-associated membrane protein 3, CEB
Anti-VAMP3 Antibody (5C283) is a Rabbit antibody targeting VAMP3. Anti-VAMP3 Antibody (5C283) can be used in WB,ICC,IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-VAMP3 Antibody (5C283) is a Rabbit antibody targeting VAMP3. Anti-VAMP3 Antibody (5C283) can be used in WB,ICC,IF,IHC,FCM. |
| Synonyms | vesicle-associated membrane protein 3, CEB |
| Ig Type | IgG |
| Clone | 5C283 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Cellubrevin on A549 (1), 293T(2) cell lysates using anti-Cellubrevin antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin. 5. ICC staining Cellubrevin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cellubrevin in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Siha cells with Cellubrevin antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Vesicle-associated membrane proteins, known as VAMPs, also designated synaptobrevins, include VAMP-1, VAMP-2, VAMP-3 (cellubrevin), and synaptotagmin, a protein that may function as an inhibitor of exocytosis. VAMP proteins are vesicular factors that are important components of the machinery controlling docking and/or fusion of secretory vesicles with their target membrane. Synaptosomal-associated proteins, known as SNAPs, including alpha- and gamma-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. Pancreatic beta-cells express VAMP-2 and VAMP-3, and either one or both of these proteins selectively control Ca2+-mediated insulin secretion. In addition, VAMP-2 and VAMP-3 are expressed on GLUT4-containing vesicle membranes isolated from 3T3-Ll adipocytes and are important components of the insulin-dependent translocation of GLUT4 to the cell surface in adipocytes. |
| Conjucates | Unconjugated |
| Immunogen | KLH conjugated synthetic peptide: within N-terminal human Cellubrevin |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 11 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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