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Anti-VAMP3 Antibody (5C283) (Synonyms: vesicle-associated membrane protein 3, CEB)

Catalog No. TMAC-00812 Copy Product Info
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Anti-VAMP3 Antibody (5C283) is a Rabbit antibody targeting VAMP3. Anti-VAMP3 Antibody (5C283) can be used in FCM,ICC,IF,IHC,WB.

Anti-VAMP3 Antibody (5C283)

Copy Product Info
🥰Excellent
Catalog No. TMAC-00812
Synonyms vesicle-associated membrane protein 3, CEB

Anti-VAMP3 Antibody (5C283) is a Rabbit antibody targeting VAMP3. Anti-VAMP3 Antibody (5C283) can be used in FCM,ICC,IF,IHC,WB.

Anti-VAMP3 Antibody
(5C283)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4987-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-VAMP3 Antibody (5C283) is a Rabbit antibody targeting VAMP3. Anti-VAMP3 Antibody (5C283) can be used in FCM,ICC,IF,IHC,WB.
Synonymsvesicle-associated membrane protein 3, CEB
Ig Type
IgG
Clone
5C283
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Cellubrevin on A549 (1), 293T(2) cell lysates using anti-Cellubrevin antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cellubrevin antibody. Counter stained with hematoxylin.
5. ICC staining Cellubrevin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Cellubrevin in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Siha cells with Cellubrevin antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundVesicle-associated membrane proteins, known as VAMPs, also designated synaptobrevins, include VAMP-1, VAMP-2, VAMP-3 (cellubrevin), and synaptotagmin, a protein that may function as an inhibitor of exocytosis. VAMP proteins are vesicular factors that are important components of the machinery controlling docking and/or fusion of secretory vesicles with their target membrane. Synaptosomal-associated proteins, known as SNAPs, including alpha- and gamma-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. Pancreatic beta-cells express VAMP-2 and VAMP-3, and either one or both of these proteins selectively control Ca2+-mediated insulin secretion. In addition, VAMP-2 and VAMP-3 are expressed on GLUT4-containing vesicle membranes isolated from 3T3-Ll adipocytes and are important components of the insulin-dependent translocation of GLUT4 to the cell surface in adipocytes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
KLH conjugated synthetic peptide: within N-terminal human Cellubrevin
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 11 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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