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Anti-VAMP1 Antibody (8U837) is a Rabbit antibody targeting VAMP1. Anti-VAMP1 Antibody (8U837) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $470 | 7-10 days | 7-10 days |
| Description | Anti-VAMP1 Antibody (8U837) is a Rabbit antibody targeting VAMP1. Anti-VAMP1 Antibody (8U837) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Clone | 8U837 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. 25 μg total protein per lane of various lysates (see on figure) probed with VAMP1 monoclonal antibody, unconjugated (TMAB-14040) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VAMP1 Monoclonal Antibody, Unconjugated (TMAB-14040) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VAMP1 Monoclonal Antibody, Unconjugated (TMAB-14040) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VAMP1 Monoclonal Antibody, Unconjugated (TMAB-14040) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with VAMP1 Monoclonal Antibody, Unconjugated (TMAB-14040) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform 1: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Isoform 3: Mitochondrion outer membrane; Single-pass type IV membrane protein. |
| Tissue Specificity | Nervous system, skeletal muscle and adipose tissue. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Involved in the targeting and/or fusion of transport vesicles to their target membrane. |
| Immunogen | A synthesized peptide: human VAMP1 |
| Antigen Species | Human |
| Gene Name | VAMP1 |
| Gene ID | |
| Protein Name | Vesicle-associated membrane protein 1 |
| Uniprot ID | |
| Function | Involved in the targeting and/or fusion of transport vesicles to their target membrane. |
| Molecular Weight | Theoretical: 13 kDa. Actual: 16 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
| Size | Quantity | Unit Price | Amount | Operation |
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