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Anti-V5 Tag Antibody (9M192)
😃Good
Catalog No. TMAY-00136
Anti-V5 Tag Antibody (9M192) is a Mouse antibody targeting V5 Tag. Anti-V5 Tag Antibody (9M192) can be used in WB,IP.
Anti-V5 Tag Antibody (9M192)
Anti-V5 Tag Antibody (9M192)
😃Good
Catalog No. TMAY-00136
Anti-V5 Tag Antibody (9M192) is a Mouse antibody targeting V5 Tag. Anti-V5 Tag Antibody (9M192) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $187 | 7-10 days | |
| 500 μL | $470 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, IP Reactivity: other |
| Ig Type | Mouse IgG1 |
| Clone | 9M192 |
| Reactivity | other |
| Verified Activity | 1. Anti-V5 Tag mouse monoclonal antibody at 1:500 dilution. -Lane A: his-ARG1-V5 transfected 293 cell Lysate(60ug) -Lane B: mFABP4-V5-his transfected 293 cell Lysate(60ug) -Lane C: V5-ARG1-his transfected 293 cell Lysate(10ug) -Lane D: V5-mFABP4-his transfected 293 cell Lysate(20ug) -Lane E: Negative control transfected 293 cell Lysate(30ug) -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions 2. V5 tag was immunoprecipitated using: -Lane A:0.5 mg V5-ARG1-His transfected 293 Whole Cell Lysate. -Lane B:0.5 mg V5-mFABP4-His transfected 293 Whole Cell Lysate. -2 µL anti-V5 tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-V5 tag mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: kDa. -Observed band size: kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 1-4 μg/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The V5 epitope tag has a 14-amino-acid sequence (GKPIPNPLLGLDST) which is derived from simian parainfluenza virus type 5 (SV5). The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Anti-V5 epitope tag antibody products recognize and detect V5-tagged proteins without a protein-specific antibody or probe in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: V5 tag sequence (GKPIPNPLLGLDST). |
| Antigen Species | other |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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